Glycopolymer-substituted gold substrates were prepared by means of living radical polymerization with a reversible addition-fragment chain transfer (RAFT) reagent. Thiol-terminated glycopolymers were bound to the gold substrate to yield the polymer-substituted interface. In the case of the gold substrate, the interactions with proteins (lectins and Shiga toxins) were analyzed by surface plasmon resonance (SPR). The interactions were highly specific and the signal-to-noise ratio of protein recognition was more than 16.
- Masayuki Toyoshima
- Tomoyuki Oura
- Yoshiko Miura
