Nature Search

Targeting and tracing antigens in live cells with fluorescent nanobodies

Ulrich Rothbauer
Kourosh Zolghadr
Heinrich Leonhardt
Research23 Oct 2006
Nature Methods

Volume: 3, P: 887-889
A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging

Nanobodies (Nbs) coupled to organic dyes are increasingly used for super-resolution cell imaging, but producing gene-specific Nbs is time-consuming. Here the authors present a peptide-tag/Nb combination for dSTORM imaging which can be easily adapted to different targets in fixed and live cells.

David Virant
Bjoern Traenkle
Ulrich Rothbauer
ResearchOpen Access02 Mar 2018
Nature Communications

Volume: 9, P: 1-14
First-in-class ultralong-target-residence-time p38α inhibitors as a mitosis-targeted therapy for colorectal cancer

Rudalska et al. describe a novel class of p38α inhibitors with increased target residence time. They explore the drugs’ specificity, pharmacokinetics and toxicity profile and show that they are efficacious in the context of colorectal cancer.

Ramona Rudalska
Jule Harbig
Daniel Dauch
ResearchOpen Access16 Jan 2025
Nature Cancer

Volume: 6, P: 259-277
Immune response to SARS-CoV-2 variants of concern in vaccinated individuals

Here, the authors characterize the antibody response from vaccinated (Pfizer BNT-162b2), infected and uninfected individuals against emerging variants of concern of SARS-CoV-2, finding reduced neutralization of a South African isolate. High IgG titers in the saliva of vaccinees suggest that transmission may be reduced.

Matthias Becker
Alex Dulovic
Nicole Schneiderhan-Marra
ResearchOpen Access25 May 2021
Nature Communications

Volume: 12, P: 1-8
Exploring beyond clinical routine SARS-CoV-2 serology using MultiCoV-Ab to evaluate endemic coronavirus cross-reactivity

Serology is an important way to monitor SARS-CoV-2 infection in the population and support vaccine development. Here the authors develop a multiplex immunoassay including spike and nucleocapsid proteins of SARS-CoV-2 and the endemic human coronaviruses with high specificity and sensitivity.

Matthias Becker
Monika Strengert
Nicole Schneiderhan-Marra
ResearchOpen Access19 Feb 2021
Nature Communications

Volume: 12, P: 1-12
Speed up to find the right ones: rapid discovery of functional nanobodies

Nanobodies have emerged as highly versatile and useful binding molecules in biomedical research. A technical report describes a cost- and time-effective in vitro platform that facilitates the generation of desired nanobodies, including conformationally selective nanobodies against agonist-bound G-protein-coupled receptors (GPCRs).

Ulrich Rothbauer
News & Views05 Mar 2018
Nature Structural & Molecular Biology

Volume: 25, P: 199-201
Decisive role of water and protein dynamics in residence time of p38α MAP kinase inhibitors

The molecular determinants of the residence time of a small molecule inhibitor at its target protein are not well understood. Here, Pantsar et al. show that the target protein’s conformational stability and solvent exposure are key factors governing the target residence time of kinase inhibitors.

Tatu Pantsar
Philipp D. Kaiser
Stefan A. Laufer
ResearchOpen Access28 Jan 2022
Nature Communications

Volume: 13, P: 1-14
Robust and durable serological response following pediatric SARS-CoV-2 infection

In this prospective cohort study, authors follow 328 households in Germany with at least one confirmed SARS-CoV-2 infection and find that children are more likely to seroconvert without symptoms and have higher specific antibody levels that persist longer than in adults.

Hanna Renk
Alex Dulovic
Roland Elling
ResearchOpen Access10 Jan 2022
Nature Communications

Volume: 13, P: 1-11
Longitudinal analysis of humoral and cellular immunity in SARS-CoV-2 exposed families

Alex Dulovic
Armin Rabsteyn
Hanna Renk
ResearchOpen Access18 Jul 2025
Scientific Reports

Volume: 15, P: 1-13
Protein-binding assays in biological liquids using microscale thermophoresis

Protein interactions in biological environments are expected to differ from the situationin vitro. In this study, a thermophoresis-based technique is described that allows the analysis of protein and small-molecule interactions in biological liquids; the work may allow more efficient drug development.

Christoph J. Wienken
Philipp Baaske
Stefan Duhr
Research19 Oct 2010
Nature Communications

Volume: 1, P: 1-7
SARS-CoV-2-derived peptides define heterologous and COVID-19-induced T cell recognition

SARS-CoV-2-specific CD4⁺ and CD8⁺ T cell epitopes are found in both convalescent patients and virus-naive volunteers and are indicative of heterologous recognition shared with seasonal cold viruses.

Annika Nelde
Tatjana Bilich
Juliane S. Walz
Research30 Sept 2020
Nature Immunology

Volume: 22, P: 74-85
Peptides in headlock – a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy

Michael B. Braun
Bjoern Traenkle
Ulrich Rothbauer
ResearchOpen Access21 Jan 2016
Scientific Reports

Volume: 6, P: 1-10
Modulation of protein properties in living cells using nanobodies

GFP fluorescence can be modulated in mammalian cells by binding to single-chain antibodies (nanobodies), selected to make GFP brighter or dimmer; these changes are explained by the crystal structures of the GFP-nanobody complexes. The applications of such nanobodies to monitor protein expression and subcellular localization in real time are explored.

Axel Kirchhofer
Jonas Helma
Ulrich Rothbauer
Research13 Dec 2009
Nature Structural & Molecular Biology

Volume: 17, P: 133-138
A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies

Bjoern Traenkle
Sören Segan
Ulrich Rothbauer
ResearchOpen Access31 Aug 2020
Scientific Reports

Volume: 10, P: 1-13
Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies

Julia Maier
Bjoern Traenkle
Ulrich Rothbauer
ResearchOpen Access21 Aug 2015
Scientific Reports

Volume: 5, P: 1-13
Akt1 and Akt3 but not Akt2 through interaction with DNA-PKcs stimulate proliferation and post-irradiation cell survival of K-RAS-mutated cancer cells

Mahmoud Toulany
Julia Maier
H Peter Rodemann
ResearchOpen Access30 Oct 2017
Cell Death Discovery

Volume: 3, P: 1-10
Wnt signaling is boosted during intestinal regeneration by a CD44-positive feedback loop

Romina J. Walter
Steffen J. Sonnentag
Véronique Orian-Rousseau
ResearchOpen Access21 Feb 2022
Cell Death & Disease

Volume: 13, P: 1-16
COVID-19 patient serum less potently inhibits ACE2-RBD binding for various SARS-CoV-2 RBD mutants

Daniel Junker
Alex Dulovic
Nicole Schneiderhan-Marra
ResearchOpen Access03 May 2022
Scientific Reports

Volume: 12, P: 1-13

Search

Filter By:

Search

Quick links