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Advanced filters: Author: Un Seng Chio Clear advanced filters

Functionalized graphene-oxide grids enable high-resolution cryo-EM structures of the SNF2h-nucleosome complex without crosslinking

Nucleosome-protein complexes stick to the air-water interface and denature upon plunge freezing for cryoEM. Here, authors Chio and Palovcak et al. develop EM grids that protect such complexes and use these grids to study the ATP-dependent chromatin remodeler SNF2h.

Un Seng Chio
Eugene Palovcak
Yifan Cheng
ResearchOpen Access12 Mar 2024
Nature Communications

Volume: 15, P: 1-12
Nucleosome density shapes kilobase-scale regulation by a mammalian chromatin remodeler

Here authors present SAMOSA-ChAAT, a method for resolving how chromatin-interacting proteins restructure individual chromatin fibers, in high throughput and at scale. They provide evidence that the imitation switch family remodeling enzymes sense nucleosome density to program internucleosomal spacing on individual molecules.

Nour J. Abdulhay
Laura J. Hsieh
Vijay Ramani
ResearchOpen Access11 Sept 2023
Nature Structural & Molecular Biology

Volume: 30, P: 1571-1581
Bi-paratopic and multivalent VH domains block ACE2 binding and neutralize SARS-CoV-2

A screening approach finds VH-domain antibodies that bind the SARS-CoV-2 Spike protein receptor-binding domain at its interface with host ACE2. Bi-paratopic and multivalent binders have high affinity and potency.

Colton J. Bracken
Shion A. Lim
James A. Wells
Research20 Oct 2020
Nature Chemical Biology

Volume: 17, P: 113-121

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