In this study, ectomesenchymal stem cells (EMSCs) were modified with the transglutaminase 2 (TG2) genes and tested for their ability to enhance bone regeneration on fibrin scaffold. In vitro analyses revealed that osteogenic differentiation of the EMSCs on the fibrin scaffold was promoted by TG2 expression. Transplanting fibrin scaffold loaded with TG2 gene-modified EMSC into bone defects on rat skull induced significantly faster bone regeneration compared with non-genetically modified EMSC.
- Wentao Shi
- Yunduan Que
- Naiyan Lu