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Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors

Cas12a-mediated cytosine (CBE) and adenine base editor (ABE) systems with elevated efficiencies and expanded target scopes are developed, by combining highly active deaminases with Cas12a variants, enCas12a, RR and RVR, recognizing non-TTTV PAMs.

Fangbing Chen
Meng Lian
Liangxue Lai
ResearchOpen Access02 Nov 2022
Communications Biology

Volume: 5, P: 1-12

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Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors

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