Nature Search

Molecular mechanics underlying flat-to-round membrane budding in live secretory cells

Shin et al. report the molecular mechanics of membrane budding: actin and dynamin pull membrane inward to form a Λ-shape profile; dynamin helices convert Λ- to Ω-shape by constricting Λ’s base, and then constrict Ω-profile’s pore to form a vesicle.

Wonchul Shin
Ben Zucker
Ling-Gang Wu
ResearchOpen Access27 Jun 2022
Nature Communications

Volume: 13, P: 1-18
Erratum: Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles

Hsueh-Cheng Chiang
Wonchul Shin
Ling-Gang Wu
Amendments and Corrections27 Jan 2015
Nature Communications

Volume: 6, P: 1-2
Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles

Fusion of vesicles with the plasma membrane generates omega-shaped intermediates that are thought to either dilate until flat or less frequently, undergo pore closure. Chiang et al.reveal that in chromaffin cells, these structures do not dilate, but change in size before pore closure or shrink until undetectable.

Hsueh-Cheng Chiang
Wonchul Shin
Ling-Gang Wu
Research24 Feb 2014
Nature Communications

Volume: 5, P: 1-17
Actin dynamics provides membrane tension to merge fusing vesicles into the plasma membrane

As vesicles fuse to the plasma membrane, they form intermediate Ω-shaped structures followed by either closure of the pore or full merging with the plasma membrane. Here Wen et al. show that dynamic actin assembly provides membrane tension to promote Ω merging in neuroendocrine cells and synapses.

Peter J. Wen
Staffan Grenklo
Ling-Gang Wu
ResearchOpen Access31 Aug 2016
Nature Communications

Volume: 7, P: 1-14
Calcium-channel number critically influences synaptic strength and plasticity at the active zone

This study uses single-channel current measurements, synaptic vesicle fusion–induced capacitance changes and structural modeling of the calyx of Held terminals to show that voltage-dependent calcium channel density at the active zone is a major determinant for the properties of neurotransmitter release and short-term synaptic plasticity.

Jiansong Sheng
Liming He
Ling-Gang Wu
Research10 Jun 2012
Nature Neuroscience

Volume: 15, P: 998-1006
Hemi-fused structure mediates and controls fusion and fission in live cells

Super-resolution imaging provides direct evidence in live cells that membrane fusion and fission are mediated through an intermediate hemi-fused structure, where fusion and calcium/dynamin-dependent fission mechanisms compete to determine the transition of the intermediate to fusion or fission.

Wei-Dong Zhao
Edaeni Hamid
Ling-Gang Wu
Research15 Jun 2016
Nature

Volume: 534, P: 548-552
Clathrin mediates membrane fission and budding by constricting membrane pores

Lisi Wei
Xiaoli Guo
Ling-Gang Wu
ResearchOpen Access11 Jun 2024
Cell Discovery

Volume: 10, P: 1-24

Search

Filter By:

Molecular mechanics underlying flat-to-round membrane budding in live secretory cells

Erratum: Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles

Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles

Actin dynamics provides membrane tension to merge fusing vesicles into the plasma membrane

Calcium-channel number critically influences synaptic strength and plasticity at the active zone

Hemi-fused structure mediates and controls fusion and fission in live cells

Clathrin mediates membrane fission and budding by constricting membrane pores

Search

Quick links