Cryomicroneedles (cryoMNs) permit the precise delivery of therapeutic cells into the skin, but are limited by the cold-chain transportation and storage. This article introduces an innovative solution to use a prefabricated porous microneedle scaffold that can be shipped at room temperature and allow on-site cell loading and usage in the clinic (i.e., the second generation cryoMNs or S-cryoMNs). The study investigates the loading and intradermal delivery of three cell types in clinically relevant in vitro and in vivo models, including mesenchymal stem cells for wound healing, melanocytes for vitiligo treatment, and antigen-pulsed dendritic cells for cancer vaccination.
- Mengjia Zheng
- Tianli Hu
- Chenjie Xu
