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Showing 1–10 of 10 results
Advanced filters: Author: Yann R. Chemla Clear advanced filters

  • UvrD is a model helicase from the non-hexameric Superfamily 1. Here, the authors use optical tweezers to measure directly the stepwise translocation of UvrD along a DNA hairpin, and propose a mechanism in which UvrD moves one base pair at a time, but sequesters the nascent single strands, releasing them after a variable number of ATP hydrolysis cycles.

    • Sean P. Carney
    • Wen Ma
    • Yann R. Chemla
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-14

  • A ring ATPase from a bacteriophage, ϕ29, helps load the dsDNA genome into the viral shell. It is shown that this motor packages the DNA in 10 base pair (bp) bursts, which are composed of four individual 2.5 bp steps, each representing hydrolysis of a single ATP. Such a non-integral step size is unexpected, and raises intriguing mechanistic questions about ATP hydrolysis within rings.

    • Jeffrey R. Moffitt
    • Yann R. Chemla
    • Carlos Bustamante
    Research
    Nature
    Volume: 457, P: 446-450

  • In several viruses and bacteriophages, DNA transport in processes such as genome packaging is dependent on a subset of the ASCE superfamily of protein enzymes consisting of multimeric ringed pumps. Little is known, however, about how these motors engage their nucleic acid substrates. Here, the genome packaging motor of the Bacillus subtilis bacteriophage is studied, revealing that the full mechanochemical cycle of the motor involves two, rather different, phases.

    • K. Aathavan
    • Adam T. Politzer
    • Carlos Bustamante
    Research
    Nature
    Volume: 461, P: 669-673

  • In viruses, multi-subunit ring-ATPases are involved in genome packaging. Here, using single-molecule techniques, the authors determine that the active bacteriophage T4 DNA packaging motor is a pentamer and show that the motor can tolerate inactive subunits, suggesting that strict coordination between the subunits is not crucial.

    • Li Dai
    • Digvijay Singh
    • Venigalla B. Rao
    ResearchOpen Access
    Nature Communications
    Volume: 12, P: 1-14

  • The combination of an ultrahigh-resolution dual optical trap with a confocal microscope allowed single-fluorophore detection of labeled oligonucleotide binding and simultaneous measurement of angstrom-scale changes in DNA tether extension.

    • Matthew J Comstock
    • Taekjip Ha
    • Yann R Chemla
    Research
    Nature Methods
    Volume: 8, P: 335-340

  • This Primer on optical tweezers describes the instrumentation and experimental designs used in most single-molecule optical tweezers assays and discusses optical tweezers measurements in systems of biophysical interest such as DNA elasticity, protein and RNA folding, and molecular motors.

    • Carlos J. Bustamante
    • Yann R. Chemla
    • Michelle D. Wang
    Reviews
    Nature Reviews Methods Primers
    Volume: 1, P: 1-29