Imaging articles from across Nature Portfolio

Hydrogen peroxide (H₂O₂) is a key signaling molecule involved in many processes that affect health and contribute to disease, but tracking its real-time dynamics inside living cells remains challenging. A new set of biosensors now enables accurate, multiparametric monitoring of H₂O₂ levels without interference from changes in pH or oxygen.

A new imaging platform combines a high-speed, multichannel camera system with an iterative spectral unmixing algorithm, enabling high-resolution imaging of up to seven distinct fluorophores, even under challenging live-cell conditions.

Cap-independently translated mRNAs could expand the scope of mRNA therapies, but yield limited protein, and existing enhancement strategies are imperfect. Here, the authors install a 5′-end modification that both boosts efficiency and facilitates in-depth characterisation of these mRNAs.

Young and colleagues describe an atmospheric pressure transmission-geometry MALDI approach equipped with plasma ionisation, which allows for subcellular mass spectrometry imaging of lipids and nucleotides in cells and tissues.

Capillary-associated macrophages are selectively lost over time, contributing to impaired vascular repair and reduced tissue perfusion in older mice.

Mapping of spatial metabolic gradients in the mouse liver and intestine identifies fructose-induced focal derangements in liver metabolism.

Brain clearance mechanisms are challenging to visualize in humans. Using magnetic resonance imaging, the authors noninvasively mapped cerebrospinal fluid motion across the brain, showing region-specific drivers in healthy participants and altered dynamics in cerebral amyloid angiopathy.

Hydrogen peroxide (H₂O₂) is a key signaling molecule involved in many processes that affect health and contribute to disease, but tracking its real-time dynamics inside living cells remains challenging. A new set of biosensors now enables accurate, multiparametric monitoring of H₂O₂ levels without interference from changes in pH or oxygen.

A new imaging platform combines a high-speed, multichannel camera system with an iterative spectral unmixing algorithm, enabling high-resolution imaging of up to seven distinct fluorophores, even under challenging live-cell conditions.

The diverse sensory input provided by physically and socially enriched environments is shown in mice to increase sensory integration and responsiveness to environmental stimuli, whereas social isolation produced the opposite effects.

A study challenges previous assumptions that the brain’s body map undergoes reorganization after limb amputation, finding that it remains remarkably stable — with implications for brain–computer interfaces and the treatment of phantom limb pain.