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Patch clamp is a type of intracellular recording that relies on attaching a flat open tip glass pipette to the outer membrane of a single cell by means of suction and then recording the activity of ion channels in the membrane.
The mechanisms controlling reproductive hormone secretion are unclear. Here the authors show that noradrenergic inputs to kisspeptin neurons are able to control pulsatile hormone release in a sex- and gonadal steroid-dependent manner.
This Protocol describes an automated high-throughput patch-clamp approach that enables the simultaneous and unbiased analysis of acutely dissociated neurons in their native state, with diverse applications ranging from the assessment of neuronal biophysics to drug development.
This protocol describes a suspended asymmetric fluid membrane. Two membrane leaflets form from different liposomes and remain accessible through microfluidic channels. Chemical and molecular reactions can be monitored optically and electrically.
In this work, the authors report that citronellal stimulates skin renewal by promoting keratinocyte proliferation through the activation of TRPV3. Structural and functional analysis elucidate that essential oil ligands activate TRPV3 channels by competitively displacing endogenous lipids from the vanilloid site. These findings can help move skin healthcare forward.
Using cryo-EM structures and electrophysiological analysis of Arabidopsis CNGC1 and CNGC5, this study characterizes plant CNGCs as a class of CNBD channels that feature Ca2+ selectivity and are not regulated by cyclic nucleotide monophosphate binding.
A deep-learning-based tool and a large ground truth dataset enable spike inference from calcium imaging data acquired in a variety of experimental conditions.
A combination of restricting channel-rhodopsin to the soma and restricting the excitation volume with temporal focusing enables the mapping of neural circuits with high precision.
