Figure 1

RT-PCR analysis of AT1a receptor, AT1b receptor and AT2 receptor mRNA in spleen and bone marrow (BM) cells of WT mice. Total RNA was isolated from spleen (Spl), BM, kidney (Kid), testis (Tes) and brain (Bra) of WT C57BL/6J mice. cDNA was synthesized and amplified for 35 cycles with each Ang II-specific primer. Expression of AT1a receptor, AT1b receptor, AT2 receptor and GAPDH are shown. RT(−) indicates that the PCR products from the RNA templates were treated with deoxyribonuclease I, but not with reverse transcriptase. A positive control reaction of an appropriate organ sample for each Ang II receptor subtype is provided at the left of each panel.