Fig. 5: Reciprocal expression of CDK1 and ID2 in human BC cells.

a–c Dot plots showing the expression of CDK1 in normal (N; n = 28) and bladder tumor (T; n = 404) samples (a), based on tumor grade (b), and pairwise gene expression correlations with CDK1 transcript expression (c) after analysis of TCGA datasets of BC patients using the GEPIA web server. Quantitative results are presented as the mean ± SEM values. b Statistical analyses were performed using the nonparametric Mann–Whitney U-test. ***p < 0.001. d Pairwise correlations between ID2 and CDK1 transcript levels in pT tumor stage subgroups in a TCGA dataset (Robertson et al., 2017) of BC patients. All gene expression data were obtained from the UCSC Xena Browser (http://xena.ucsc.edu/). e–g RT-qPCR (e) and western blot (f, g) analyses of the expression of ID2 in basal HT1197 or luminal HT1376 MIBC cells after exposure to 20 μM RO-3306 for 2 h or 40 μM apigenin for 24 h. β-Actin was used as the loading control. Molecular weight (MW) marker sizes (in kD) are shown on the left. g Quantification of ID2 protein expression. e, g Quantitative data are expressed as the mean ± SEM values (n = 4). Statistical analyses were performed using one-way ANOVA with the Bonferroni post hoc test. ***p < 0.001 compared with the nontreated (NT) control group.