Fig. 7: Transcriptome changes induced by overexpression of Nanog in NPCs.
From: Induction of Nanog in neural progenitor cells for adaptive regeneration of ischemic brain
NPCs obtained from the cerebellum of postnatal day 4 mice were transduced with Nanog using a retroviral vector (MIG). Five days after transduction, RNA was purified and subjected to transcriptome analysis by RNA-seq and subjected to by Gene Ontology and KEGG analysis. a Heatmap showing two-way hierarchical clustering analysis (Euclidean method, complete linkage). Differentially expressed genes (DEGs) were classified by lpe.p < 0.05 and fold change >1.5 between three batches of control (MIG 1–3) and Nanog-transduced cells (Nanog 1–3) (n = 3). b Results of the 20 most enriched terms in the GO functional biological process analysis. Each enriched GO term is shown with adjusted p values and the intersection size (gene ratio). c KEGG pathway analysis of DEGs between MIG- and Nanog-transduced cells. Significantly (FDR <0.05) enriched signaling pathways are shown.
