Fig. 1: LJ-4378 treatment increases lipolysis and mitochondrial content of adipocytes in a dose-dependent manner. | International Journal of Obesity

Fig. 1: LJ-4378 treatment increases lipolysis and mitochondrial content of adipocytes in a dose-dependent manner.

From: Anti-obesity effects of the dual-active adenosine A2A/A3 receptor-ligand LJ-4378

Fig. 1: LJ-4378 treatment increases lipolysis and mitochondrial content of adipocytes in a dose-dependent manner.The alternative text for this image may have been generated using AI.

a The chemical structure of LJ-4378; (2R,3S,4R)-2-(6-Amino-2-(hex-1-ynyl)-9H-purin-9-yl)- tetrahydrothiophene-3,4-diol. b Cytotoxicity effects of LJ-4378 on brown adipocytes (BAs) at indicated concentration (24 h treatment, n = 5, means ± SEM, ****p < 0.0001). c Immunoblot analysis of hormone-sensitive lipase (HSL) and phosphorylated HSL (p-HSL, serine 660) of BAs treated with LJ-4378 for 4 h (n = 5, means ± SEM, *p < 0.05, ***p < 0.001, ****p < 0.0001). d Free fatty acid (FFA) levels in conditioned media of BAs treated with 0.1 μM LJ-4378 for 24 h (n = 5, means ± SEM, ****p < 0.0001). e Immunoblot analysis of BAs treated with LJ-4378 at indicated concentration for 48 h (n = 5, means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).

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