Fig. 3: HNRNPA2B1 regulates the alternative splicing and promotes invasive behavior of head and cancer cells.

Semiquantitative PCR to analysis of HNRNPA2B1 target gene. a WWOX exon 6–8 inclusion/skipping. b CFLAR exon 7 inclusion/skipping. c Pictorial diagram to show differential splicing pattern of MST1R in HNC cells. d MST1R exon 11 inclusion/skipping. e MST1R-minigene transfection and fluorescence analysis in control and HNRNPA2B1-depleted H413 HNC cells, oral cancer cells. Scale bar = 200 µm. f Immunoblotting of HNRNPA2B1, pAkt, pan Akt, E-cadherin, vimentin, and GAPDH in control and HNRNPA2B1-depleted H413 cells.