Fig. 6: BAPTA-AM (BA) attenuated the intracellular calcium changes in astrocytes in response to KA in the left co-culture compartment and prevented neurite beading.
a Fluo-4-direct (F − F0/F0) in astrocytes of the astrocytes-only compartment at baseline (time = 0), and maximum (time = peak), representing calcium changes following vehicle, KA or BA treatment. Scale bar = 25 µm. b Changes in fluo-4-direct, representing free intracellular calcium (F − F0/F0) in intracellular calcium levels following KA treatment (15-min) to the left co-culture compartment, or BA (15-min) to the astrocyte-only compartment. *p < 0.05; one-way ANOVA with Tukey’s post hoc test. n = 3 (mean ± SEM). c Beaded axons (%) in a ROI (mm2) of each co-culture compartment at 6 h following KA treatment (15-min) to the left co-culture compartment ***p < 0.001; one-way ANOVA with Tukey’s post hoc test. n = 3 (mean ± SEM). d Beaded axons (%) in a ROI (mm2) of each co-culture compartment at 6 h following 15-min BA treatment to the astrocyte-only compartment. ***p < 0.001; one-way ANOVA with Tukey’s post hoc test. n = 3 (mean ± SEM). e Beaded axons (%) in a ROI (mm2) of each co-culture compartment at 6 h following 15-min BA treatment to the astrocyte-only compartment prior to KA treatment (15-min) to the left co-culture compartment. ns non-significant; one-way ANOVA with Tukey’s post hoc test. n = 3 (mean ± SEM). f Beaded axons (%) in a ROI (mm2) of each co-culture compartment in KA-treated co-cultures 6 h following BA treatment to the astrocyte-only compartment, and 6 h following BA treatment to the astrocyte-only compartment prior to KA treatment to the left co-culture compartment. ns non-significant; one-way ANOVA with Tukey’s post hoc test. n = 3 (mean ± SEM)
