Fig. 6: EphrinB2-Fc-His and Fc-TNYL-RAW-GS reduce local tumor growth and distant metastasis in C57BL/6 mice implanted with MOC2 cells.

A Experimental design for C57BL/6 J mice implanted with MOC2 WT cancer cells and treated with different plasmids (n = 10 per group). Hydrodynamic tail vein injections (HTVI) and radiation therapy (8 Gy) were administered as indicated. Mice treated with RT alone or Sleeping Beauty (SB) + RT served as controls. B Average tumor volume curves comparing effects of different plasmids on local tumor growth. C Dot plot showing the effects of different plasmids on tumor volume 30 DPI. D, E Kaplan-Meier curves showing therapeutic effects of different plasmids on overall survival (D) and lung metastasis free survival (E) in MOC2 WT implanted C57BL/6 mice. For Kaplan-Meier curves showing lung metastasis free survival, numbers at risk indicate mice that were alive without metastases at specified timepoints. F Contingency table quantifying the incidence of lung metastasis detected by CT scans in MOC2 WT implanted mice by 35 DPI. The experiment was replicated twice. Comparison of tumor volume between the control and experimental groups was done using a Dunnett post hoc test after one-way ANOVA was performed. For Kaplan-Meier survival curves, significance was determined by a log-rank Mantel-Cox test. For contingency tables indicating the incidence of metastases, significance was determined by a Chi-square test. Significance was determined if the p-value was < 0.05*, < 0.01**, < 0.001***, and < 0.0001****. p-values are indicated for the figures (C) RT vs EFNB2-Fc-His ***p = 0.0003; RT vs Fc-TNYL-RAW-GS ***p = 0.0002; RT vs SB + RT ns p = 0.3757, (D) *p = 0.0485, (E) RT vs EFNB2-Fc-His **p = 0.0075; RT vs Fc-TNYL-RAW-GS *p = 0.0488, F **p = 0.0098. The error bars represent the standard error of the mean ( ± SEM).