Fig. 5: M2 macrophages mediated by MS4A4A regulate malignant behavior and TMZ resistance of GBM cells. | Oncogene

Fig. 5: M2 macrophages mediated by MS4A4A regulate malignant behavior and TMZ resistance of GBM cells.

From: MS4A4A promotes macrophages M2 polarization via NF-κB /JAK-STAT6 axis, resulting GBM malignant progression

Fig. 5: M2 macrophages mediated by MS4A4A regulate malignant behavior and TMZ resistance of GBM cells.The alternative text for this image may have been generated using AI.

A The scheme of the CM derived from macrophages with different MS4A4A/Ms4a4a expression levels used to further treat GBM cells. B CCK-8 assays showing the proliferation of GBM cells treated with the CM from different macrophages (n = 3). C Transwell assays showing the migration of GBM cells treated with the CM from different macrophages (n = 3). The histogram displays the statistical results of the migration analysis. D, E. Cell scratch assay of GBM cells treated with the CM from different macrophages (n = 3). The histogram displays the statistical results of the migration analysis. F, G Apoptosis of GBM cells treated with the CM from different macrophages under TMZ treatment. The histogram displays the statistical results of the apoptosis analysis. H, I Comet assay performed with GBM cells treated with the CM from different macrophages under TMZ treatment. The boxplots show the statistical results of comet assays performed with GBM cells treated with the CM from different macrophages under TMZ treatment (n = 50). Data, mean ± SD. P values were determined by Student t test or two-way ANOVA followed by Tukey’s multiple comparison test. Significant results are presented as **, P < 0.01 or ***, P < 0.001.

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