Fig. 2
Increased M1 macrophages and decreased M2 macrophages lead to a reduced megakaryopoiesis-supporting ability in the bone marrow of PT patients. a Representative images (left panel, scale bars represent 50 μm) and quantification (right panel) of the phagocytosis assays for the cultivated BM MФs from PT patients, GGF patients and HD after 7 days in culture. b Representative images (left panel, scale bars represent 50 μm) and quantification (right panel) of the transwell migration assays for the cultivated BM MФs from PT patients, GGF patients, and HD after 7 days in culture. The intracellular levels of c TNF-ɑ and d TGF-β were analyzed in BM MФs from PT patients and GGF patients after 7 days of culture. MK production and maturation, colony-forming unit MK (CFU-MK) plating efficiencies, and platelet release were analyzed after coculture with the cultivated BM MФs from PT patients and GGF patients. The e MK count, f CFU-MK count (left panel: representative images, scale bars represent 50 μm; right panel: quantification), g platelet count and h MK ploidy distribution were analyzed after 12 days of coculture. Data are presented as the means ± SEM (*P ≤ 0.05, **P ≤ 0.01)
