Fig. 1
Treatment with nimesulide, diclofenac and exisulind, but not ketoprofen increase p53 acetylation at K382 residue. Data from more NSAIDs are shown in Fig. S1A. Immunoblot analysis was carried out using anti-acetyl (K382) p53 and anti-total p53 antibodies and protein extracts obtained from MDA-MB-231 cells in time course experiments (a) or after 3 h treatment with increasing concentrations (b) of the indicated NSAIDs. For the time course experiments, cells were treated with 90 μM for each compound. Data are represented as mean ± SEM. Bars in the graphs represents densitometry quantifications of the autoradiographic signals (acetylated p53 vs total p53); β-actin is reported as loading control. *P < 0.05 **P < 0.01 ***P < 0.001 ****P < 0.0001 versus the baseline level; P-values were calculated by one-way ANOVA followed by Bonferroni’s test
