Fig. 3: Influence of IFP design on immunological synapse formation.
From: Rational design of PD-1-CD28 immunostimulatory fusion proteins for CAR T cell therapy
a IFP-transduced T cells and CAR- and IFP-transduced T cells were cocultured with WT and PD-L1+ Nalm-6 tumour cells, and functional conjugate formation was analyzed. b T cells in functional conjugates with tumour cells expressed by F-Actin (AF647) polarisation have been quantified in 3 to 9 independent samples per condition, the experiment is representative of 2 independent repetitions with different donors. c T cells in functional conjugation had their synapse area measured [µm], each dot depicts one T cell in conjugate, representative of 2 experiments with different donors. P values for (b and c) are based on a two-tailed unpaired t-test. Statistical analysis in (c) between CAR PTM and CAR CTM + 41EC, CAR PTM and CAR CTM + 39EC, CAR PTM and CAR CTMΔ12EC resulted in non-significant differences.
