Fig. 6: The circ-GRB10/miR-328-5p axis is critical for cell function. | Cell Death & Disease

Fig. 6: The circ-GRB10/miR-328-5p axis is critical for cell function.

From: Circular RNA GRB10 as a competitive endogenous RNA regulating nucleus pulposus cells death in degenerative intervertebral disk

Fig. 6: The circ-GRB10/miR-328-5p axis is critical for cell function.The alternative text for this image may have been generated using AI.

a The Venn diagram indicates the relevant target genes mediated by miR-328-5p. ERBB2 was intersected and predicted by three different databases. b NP cells from control tissues were transfected with miR-328-5p with or without circ-GRB10 overexpress plasmid. qRT-PCR was used to detect the relative mRNA levels of ERBB2 compared with controls. c Relative protein levels of ERBB2 when transfected with miR-328-5p mimics and reversed by circ-GRB10 expression plasmid. d circ-GRB10 siRNA with or without miR-328-5p inhibitor was transfected into NP cells from control tissues and the mRNA level of ERBB2 was evaluated by qRT-PCR. e Western blot analysis of ERBB2 protein level following treatment of NP cells from control tissues with circ-GRB10 siRNA or miR-328-5p inhibitor. GAPDH was used as control. *P < 0.05, **P < 0.01, n = 3

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