Fig. 2: Morphological features of DPSC growth and flow cytometric analyses with DPSC markers. | Cell Death & Disease

Fig. 2: Morphological features of DPSC growth and flow cytometric analyses with DPSC markers.

From: Therapeutic effect of dental pulp stem cell transplantation on a rat model of radioactivity-induced esophageal injury

Fig. 2: Morphological features of DPSC growth and flow cytometric analyses with DPSC markers.The alternative text for this image may have been generated using AI.

a Primary DPSCs assumed a fibroblast-like appearance on the first day (×40). b A large number of spindle cells attached to the culture dish on the fourth day (×40). c The majority (80–90%) of the DPSCs were confluent after 2 weeks (×40). d On days 1–3, the P1, P3, and P4 DPSCs proliferated slowly, and the cells appeared to proliferate rapidly from day 4, entering the logarithmic phase. The increase in the number of cells then slowed gradually, reaching the plateau phase on day 8. e, f The DPSCs differentiated into the osteogenic and adipogenic lineages (×100). g A total of 99.85% of the cells reacted with anti-rat CD29; 89.08% of the cells reacted with anti-rat CD90; 1.42% of the cells reacted with anti-rat CD45; and 0.12% of the cells reacted with anti-rat CD34

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