Fig. 5: Trehalose and rapamycin differently modify LC3/mitochondria and p62/mitochondria colocalization in docetaxel-treated PC3 cells.

a PC3 cells were treated for 48 h with 100 mM trehalose or 100 nM rapamycin and/or 20 nM docetaxel. The cells were stained with 250 nM MitoTracker Orange for 30 min to stain the mitochondria and then fixed with paraformaldehyde. IF shows LC3 and mitochondria localization. Nuclei were stained with DAPI. b PC3 cells were treated for 48 h with 100 mM trehalose or 100 nM rapamycin and/or 20 nM docetaxel. The cells were stained with 250 nM MitoTracker Orange for 30 min to stain the mitochondria and then fixed with paraformaldehyde. IF shows p62 and mitochondria localization. Nuclei were stained with DAPI. Images were acquired by Zeiss Axiovert 200 microscope equipped with ×63/1.4 objective lens linked to a Coolsnap Es CCD camera (Ropper Scientific-Trenton, NJ, USA), scale bar 20 μm