Fig. 2: Isogenic iPSCs and NT-ESCs show similar alveolar epithelial cell (AEC) differentiation potentials. | Cell Death & Disease

Fig. 2: Isogenic iPSCs and NT-ESCs show similar alveolar epithelial cell (AEC) differentiation potentials.

From: Reprogramming mechanisms influence the maturation of hematopoietic progenitors from human pluripotent stem cells

Fig. 2: Isogenic iPSCs and NT-ESCs show similar alveolar epithelial cell (AEC) differentiation potentials.The alternative text for this image may have been generated using AI.

a Schematic diagram of serum- and feeder-free multistep AEC differentiation from human iPSCs and NT-ESCs. DE definitive endoderm, AFE anterior foregut endoderm. b Immunofluorescence staining for CPM, NKX2.1, and EPCAM in AECs on day 14 of differentiation. Scale bar, 200 μm. c, d Flow cytometry analysis of cells harvested on days 14 and 25 showing the frequencies of specific markers for VAFE and ADAE cells, respectively. The induction efficiency of VAFE and ADAE cells was determined by measuring the percentages of CPM(+), NKX2.1(+), EPCAM(+), and SFTP-B(+) cells by flow cytometry on days 14 and 25, respectively. e AEC differentiation was measured by gradually reducing OCT4 and increasing the lineage specific markers (GATA6 and NKX2.1). The relative expression levels of target genes are normalized to GAPDH in each well. Values are relative to day 0 (day 0 = 1). f Heat maps representing the RNA expression patterns of genes associated with lung development in isogenic iPSCs and NT-ESCs. g Boxplots representing the mean lung development-related gene promoter methylation levels in each isogenic pair. All bars indicate the mean±SD from three independent experiments

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