Fig. 2: BET inhibitors reduce the constitutive and IFN-γ-induced expression of IDO1. | Cell Death & Disease

Fig. 2: BET inhibitors reduce the constitutive and IFN-γ-induced expression of IDO1.

From: Inhibition of the BET family reduces its new target gene IDO1 expression and the production of l-kynurenine

Fig. 2: BET inhibitors reduce the constitutive and IFN-γ-induced expression of IDO1.The alternative text for this image may have been generated using AI.

The protein levels of IDO1 were detected by western blotting in human tumor cell lines derived from different tissues (a), in Ty-82, SKOV3, and MDA-MB-231 cells treated with JQ1, OTX015, or NLG919 at 1 μM for 24 h (b), in SKOV3, Capan-1, and A549 cells that were treated with IFN-γ (10 ng/ml), JQ1 (1 μM), OTX015 (1 μM), or their respective combinations for 24 h (c), in SKOV3 (d) and A549 (e) cells treated with JQ1 (1 μM) [d(a)] or IFN-γ (10 ng/ml, 24 h) plus JQ1 (1 μM) [e(a)] for the indicated time or with gradient concentrations of JQ1 [d(b)] or JQ1 plus IFN-γ (10 ng/ml) [e(b)] for 24 h. GAPDH was used as the loading control. The relative mRNA levels of IDO1 were determined by RT-qPCR in Ty-82, SKOV3, and MDA-MB-231 cells treated with JQ1 or OTX015 at 1 μM for 24 h (f), in SKOV3 cells treated with JQ1 (1 μM) for the indicated time (g) or with gradient concentrations of JQ1 for 24 h (h), or in A549 cells treated with IFN-γ (10 ng/ml) plus JQ1 or OTX015 (1 μM) for 24 h (i), with IFN-γ (10 ng/ml) plus gradient concentrations of JQ1 for 24 h (j), or with IFN-γ (10 ng/ml, 24 h) plus JQ1 (1 μM) for the indicated time (k). All the data were from three independent experiments, and if applicable, were expressed as mean ± SD (error bar); *p < 0.05; **p < 0.01; ***p < 0.001; + , treated with the indicated drug

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