Fig. 3: TRPA1 mediates cell death and oxidative stress.
From: TRPA1 mediates damage of the retina induced by ischemia and reperfusion in mice
a Representative images and mean gray value of the active caspase-3 staining in retina from Trpa1+/+ and Trpa1−/− mice after ischemia and reperfusion (I/R). b Representative images and mean gray value of 4-HNE staining in GCL and INL in retina from C57BL/6J mice after I/R and treated daily with eye drops (5 µl, 10 mM) α-lipoic acid (α-LA) or its vehicle (Veh). c Representative images of hematoxylin and eosin staining, retinal thickness and total number of cells in the GCL in retina from C57BL/6J mice after I/R and treated daily with eye drops (5 µl, 10 mM) α-LA or its Veh. d, e Representative images and mean gray value of 4-HNE staining in GCL and INL in retina from (d) Trpa1+/+ and Trpa1−/− after I/R and (e) C57BL/6J mice after I/R and treated daily with eye drops (5 µl, 10 mM) A-967079 (A96) or its Veh. All data are from retinas collected at day-7 after I/R. Control (CTL) indicates mice receiving all the procedures except I/R. Scale bars: 20 µm (a, b, d, e); 50 µm (c). Data are expressed as the percentage difference (Δ%) from CTL, and displayed as mean ± SEM, n = 4 to 5 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 between indicated groups; two-way ANOVA and Bonferroni post hoc test.
