Fig. 2: Dynamic changes of the expression of NLRP3 inflammasome components were inversely proportional to TRAF3 expression.

a JM1, SU-DHL-4, and primary B cells were treated with BAFF (200 ng/ml) for the indicated periods of time. cIAP1 in cell lysates was immunoprecipitated with cIAP1 antibody and protein G beads. Co-precipitated TRAF2, TRAF3, pro-caspase-1, NLRP3, and ASC proteins were detected by immunoblot. b Lymphoma or primary B cells were treated with BAFF at various concentrations for 2-h. The co-precipitated proteins with cIAP1 were analyzed as in a by Western blot. c B cells were transfected using lipofectamine with scrambled siRNA as controls (indicated by −) and siRNA that targets cIAP1 (0.5 nM) and cIAP2 (0.5 nM), prior to BAFF treatments for 24-h. Caspase-1 cleavage was analyzed by Western blot. Cell-based studies were performed at least three times, and showed similar findings. The knockdown effects of cIAP1/2 siRNA were analyzed using real-time PCR at 24-h post-transfection (data not shown).