Fig. 5: Knockout of the LMNB2 gene can inhibit tumor formation in colorectal cancer cells.
From: LMNB2 promotes the progression of colorectal cancer by silencing p21 expression
A Western blotting confirmed that the LMNB2 gene was knocked out in the HCT116 stable cell line at the protein level. B Gross observation of subcutaneous tumors of nude mice on HCT116 cells transfected with shLMNB2 and shCtrl lentivirus (10 mice per group). The mice were killed 20 days after injection and the transplanted tumors were collected. C Detection of LMNB2 protein levels in five cancer tissues and paired normal colon tissues by western blotting. D Analyze the weight of the transplanted tumor. E The volume of transplanted tumor in nude mice. The calculation formula of transplanted tumor volume is: V = (L × W2)/2. F Tumor sections were immunohistochemically stained with antibodies against LMNB2, p21, and Ki67, and a representative image was displayed (magnification: ×400). *P < 0.05. L represents the long axis, W represents the short axis.
