Fig. 4: The regulation by STAT4 of CYP2E1. | Cell Death & Disease

Fig. 4: The regulation by STAT4 of CYP2E1.

From: Stat4 rs7574865 polymorphism promotes the occurrence and progression of hepatocellular carcinoma via the Stat4/CYP2E1/FGL2 pathway

Fig. 4: The regulation by STAT4 of CYP2E1.The alternative text for this image may have been generated using AI.

A–D The correlation between content of STAT4 and activity of CYP2E1 (Vmax and Clint) was analyzed. The result revealed that content of STAT4 was significantly positively correlated with the Vmax and Clint in control and HCC liver tissues (N = 42, R and P-values by Pearson’s correlation test are depicted). E, F The expression of STAT4 and CYP2E1 in HepG2 and L02 cells in the IL-12 treated group and the STAT4 siRNA group by western blot (N = 3). GAPDH in each lane served as an internal control for normalization. HepG2 and L02 cells for 48 h with blank control group, negative control group, IL-12 group, and siRNA-STAT4 group. Data are representative of three independent experiments. G The effect of STAT4 on CYP2E1 promoter activity was detected by double luciferase reporter gene. Promoter-NC + TFs: STAT4 (TFs) and CYP2E1 promoter negative control plasmid; (CYP2E1 promoter + TFs-NC group): CYP2E1 promoter and STAT4-negative control plasmid (TFs-NC) group; CYP2E1 promoter-NC and TFs-NC plasmid group; CYP2E1 promoter and TFs (STAT4); Promoter: CYP2E1 promoter normal plasmid group (N = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 was assessed by one-way ANOVA.

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