Fig. 2: SIKs were not the targets of HG-9-91-01 for blockage of TNF- and TLR-induced necroptosis. | Cell Death & Disease

Fig. 2: SIKs were not the targets of HG-9-91-01 for blockage of TNF- and TLR-induced necroptosis.

From: Salt-inducible kinases inhibitor HG-9-91-01 targets RIPK3 kinase activity to alleviate necroptosis-mediated inflammatory injury

Fig. 2: SIKs were not the targets of HG-9-91-01 for blockage of TNF- and TLR-induced necroptosis.The alternative text for this image may have been generated using AI.

a Peritoneal macrophages were transfected with indicated siRNA for 3 days. Knockdown efficiency was determined by RT-PCR. Bars represent the mean ± SEM from at least three independent experiments. Peritoneal macrophages were transfected with indicated siRNA for 3 days and then treated with TNF + zVAD (b), LPS + zVAD (c), or poly I:C + zVAD (d) for 12 h. Cell death was determined by released LDH. Bars represent the mean ± SEM from at least three independent experiments performed in duplicate. e, f Peritoneal macrophages were transfected with specific siRNA targeting CRTC3 for 3 days and then treated with TNF + zVAD for 12 h. e Knockdown efficiency was determined by RT-PCR. f Cell death was determined by released LDH. Bars represent the mean ± SEM from at least three independent experiments performed in duplicate. Peritoneal macrophages were pretreated with YKL or HG for 30 min and then treated with TNF (g), LPS (h), poly I:C (i) or zVAD for 12 h. Cell death was determined by released LDH. Bars represent the mean ± SEM from at least three independent experiments performed in triplicate. Student’s t test, *p < 0.05, **p < 0.01, ***p < 0.001.

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