Fig. 3: mTORC2-mediated signaling is critical for the migration of γδ T cells into the injured liver by increasing chemokine receptor expression. | Cell Death & Disease

Fig. 3: mTORC2-mediated signaling is critical for the migration of γδ T cells into the injured liver by increasing chemokine receptor expression.

From: IL-1β-activated mTORC2 promotes accumulation of IFN-γ+ γδ T cells by upregulating CXCR3 to restrict hepatic fibrosis

Fig. 3: mTORC2-mediated signaling is critical for the migration of γδ T cells into the injured liver by increasing chemokine receptor expression.The alternative text for this image may have been generated using AI.

a Representative histograms and b MFI of p-S6 (ser235/236) and p-AKT (pS473) on γδ T, Vγ1 and Vγ4 cell subsets in the liver form Oil or CCl4-treated mice. CD45+ CD3e+ TCRδ+ cells were gated. c RNAseq heatmap of differentially expressed genes in samples of Ricf/f (n = 6 mice per sample), RicKO (n = 5 mice per sample) γδ T cells isolated from pooled spleens. Expression of chemokine receptor genes for γδ T cells was shown. d The MFI was determined by flow cytometry showing the expression of CCR2, CCR4, CCR5, CCR6, CXCR3, CXCR5 and CXCR6 molecule expression on γδ T cells from Ricf/f and RicKO mice. e–i TCRδ-/- mice were reconstituted with Ricf/f Vγ4 or RicKO Vγ4 cells, and repetitive CCl4 were challenged twice weekly for 4 weeks. e Representative liver histology of H&E, Sirius Red staining and Masson’s Trichrome staining. f Sirius Red staining and Masson’s Trichrome staining were quantified by ImageJ. g Serum ALT and AST levels. h Hydroxyproline content in liver tissues. i qRT-PCR analysis of the relative expression of Col1α1, Acta2, TIMP-1 and MMP-9 in mouse liver. j, k Representative western bolt images and quantitative analysis of α-SMA expression in liver tissues. l Representative FACS plots, statistical analysis of the percentage and the absolute cell number of γδ T cells in the liver. m Experimental scheme. Vγ4 cells from CD45.1(WT) and CD45.2 mice (Ricf/f or RicKO) were isolated and mixed at a ratio of 1:1 and injected (i.v.) into TCR δ-/- hosts, and then host mice were injected (i.p) with a single dose of CCl4 for 24 h to induce liver injury. n Representative FACS plots of CD45.1+ Vγ4 and CD45.2+ Vγ4 cells were shown, and the percentages of donor-derived Vγ4 cells in the liver of the recipient mice were calculated. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 in comparison with the corresponding controls, by unpaired Student’s t-test between two groups.

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