Fig. 4: IL-1β-induced γδ T cell mTOR-signaling activation and chemokine receptor expression. | Cell Death & Disease

Fig. 4: IL-1β-induced γδ T cell mTOR-signaling activation and chemokine receptor expression.

From: IL-1β-activated mTORC2 promotes accumulation of IFN-γ+ γδ T cells by upregulating CXCR3 to restrict hepatic fibrosis

Fig. 4: IL-1β-induced γδ T cell mTOR-signaling activation and chemokine receptor expression.The alternative text for this image may have been generated using AI.

a Serum was collected at the indicated time points after CCl4 treatment (n = 5/group; 3 replicates), and serum levels of IL-1β were determined by using enzyme-linked immunosorbent assay (ELISA) kits according to manufacturer’s instructions. b Statistical analysis of the percentage and the absolute cell number of γδ T cells in liver tissue at indicated time points after CCl4 treatment. c–e Whole spleen cell suspensions from C57BL/6 WT mice were stimulated with or without IL-1β (10 ng/mL) for 30 min, c representative histograms and d MFI of p-S6 (ser235/236) and p-AKT (pS473) in γδ T, Vγ1 and Vγ4 cell subsets in IL-1β treated splenocytes. e The MFI was determined by flow cytometry showing the expression of CCR2, CCR4, CCR5, CCR6, CXCR3, CXCR5 and CXCR6 on γδ T cells in IL-1β treated splenocytes. Data are shown as mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001 in comparison with the corresponding controls, by unpaired Student’s t-test between two groups.

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