Fig. 2: SQLE promotes PC cell proliferation in vitro. | Cell Death & Disease

Fig. 2: SQLE promotes PC cell proliferation in vitro.

From: SQLE promotes pancreatic cancer growth by attenuating ER stress and activating lipid rafts-regulated Src/PI3K/Akt signaling pathway

Fig. 2: SQLE promotes PC cell proliferation in vitro.The alternative text for this image may have been generated using AI.

A Western blotting images showing SQLE protein levels in normal pancreatic ductal epithelial cell HPNE and PC cell lines including PANC-1, MIA PaCa-2, BxPC-3, AsPC-1, and T3M4. B Knockdown of SQLE protein levels of T3M4 and AsPC-1 cells were confirmed by Western blotting analysis. C, D Cell growth curves of T3M4 and AsPC-1 cells expressing control or SQLE shRNA. E, F Representative images of EdU staining (left panel) and EdU positive ratio (right panel) of T3M4 and AsPC-1 cells expressing control or SQLE shRNA. Scale bar: 100 μm. G Colony formation of T3M4 and AsPC-1 cells expressing control or SQLE shRNA. H Overexpression of SQLE protein levels of PANC-1 and MIA PaCa-2 cells were confirmed by Western blotting analysis. I, J Cell growth curves of PANC-1 and MIA PaCa-2 cells expressing vector or SQLE overexpression. K, L Representative images of EdU staining (left panel) and EdU positive ratio (right panel) of PANC-1 and MIA PaCa-2 cells expressing vector or SQLE overexpression. Scale bar: 100 μm. M, N Colony formation of PANC-1 and MIA PaCa-2 cells expressing vector or SQLE overexpression. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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