Fig. 7: SQLE inhibitors terbinafine and NB-598 suppress proliferation, induce cell cycle arrest and activate ER stress-triggered apoptosis of PC cells in vitro. | Cell Death & Disease

Fig. 7: SQLE inhibitors terbinafine and NB-598 suppress proliferation, induce cell cycle arrest and activate ER stress-triggered apoptosis of PC cells in vitro.

From: SQLE promotes pancreatic cancer growth by attenuating ER stress and activating lipid rafts-regulated Src/PI3K/Akt signaling pathway

Fig. 7: SQLE inhibitors terbinafine and NB-598 suppress proliferation, induce cell cycle arrest and activate ER stress-triggered apoptosis of PC cells in vitro.The alternative text for this image may have been generated using AI.

A Cell growth curves of AsPC-1 cells treated with 0, 25, 50, and 100 μM terbinafine for 4d. B Cell growth curves of AsPC-1 cells treated with 0, 1, 5, and 10 μM NB-598 for 4d. C Cell cycle analysis of AsPC-1 cells treated with 0, 25, 50, and 100 μM terbinafine for 48 h. D Cell cycle analysis of AsPC-1 cells treated with 0, 1, 5, and 10 μM NB-598 for 48 h. E Cell apoptosis analysis of AsPC-1 cells treated with 0, 50, and 100 μM terbinafine for 48 h. F Cell apoptosis analysis of AsPC-1 cells treated with 0, 5, and 10 μM NB-598 for 48 h. G Detection of formation of lipid droplets using BODIPY 493/503 staining in AsPC-1 cells treated with 0 and 50 μM terbinafine for 48 h. Left panel, representative images of BODIPY staining. Right panel, statistical analysis of relative fluorescence intensity of BODIPY staining. Scale bar: 100 μm. H Detection of formation of lipid droplets using BODIPY 493/503 staining in AsPC-1 cells treated with 0 and 5 μM NB-598 for 48 h. Left panel, representative images of BODIPY staining. Right panel, statistical analysis of relative fluorescence intensity of BODIPY staining. Scale bar: 100 μm. I Western blotting analysis showing proteins involved in the ER stress pathway in AsPC-1 cells treated with 50 μM terbinafine (left panel) and 5 μM NB-598 (right panel) for 0, 8, 16, 24, 32, and 48 h. J Western blotting analysis showing the expression of Src, p-Src, PI3K, p-PI3K, Akt, p-Akt, Erk, and p-Erk in AsPC-1 cells treated with 0, 50, and 100 μM terbinafine for 48 h. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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