Fig. 2: PDIA6 promotes cell proliferation and metastasis in endometrial cancer. | Cell Death & Disease

Fig. 2: PDIA6 promotes cell proliferation and metastasis in endometrial cancer.

From: PDIA6, which is regulated by TRPM2-AS/miR-424-5p axis, promotes endometrial cancer progression via TGF-beta pathway

Fig. 2: PDIA6 promotes cell proliferation and metastasis in endometrial cancer.The alternative text for this image may have been generated using AI.

A CCK8 assay was performed to detect the cell proliferation after PDIA6 knockdown in Ishikawa and AN3CA cells. B Colony formation assay proved that the proliferation of Ishikawa and AN3CA cells were inhibited after PDIA6 knockdown. C Transwell assay and wound healing assay confirmed that knockdown of PDIA6 in Ishikawa and AN3CA cells diminishes metastatic ability. D Representative images of xenograft tumors in the PDIA6 knockdown and control groups after subcutaneous injection of Ishikawa cells. E Tumor volumes was calculated after injection every 5 days and tumor growth curves were conducted. F Tumor weights were lower for xenograft tumors with PDIA6 knockdown than for xenograft tumors with control groups. G Cell viability was detected by colony formation assay while Ishikawa and AN3CA cells were transfected with NC and PDIA6-OE. H EDU assay were performed to identify proliferation after PDIA6 overexpressed in Ishikawa and AN3CA cells. I Metastatic ability in Ishikawa and AN3CA cells after PDIA6 overexpression were measured by Transwell assay and wound healing assay. J Representative pictures of PDIA6 overexpression group and control xenograft tumors after subcutaneous injection of Ishikawa cells. K, L Tumor volume and weight were higher for xenograft tumors with PDIA6 overexpression (PDIA6-OE) than controls (NC).

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