Fig. 1: Frataxin is in close proximity to mitochondrial respiratory chain complexes I, II and III in healthy hiPSC-derived CMs and hiPSC-derived NPCs. | Cell Death & Disease

Fig. 1: Frataxin is in close proximity to mitochondrial respiratory chain complexes I, II and III in healthy hiPSC-derived CMs and hiPSC-derived NPCs.

From: Human frataxin, the Friedreich ataxia deficient protein, interacts with mitochondrial respiratory chain

Fig. 1: Frataxin is in close proximity to mitochondrial respiratory chain complexes I, II and III in healthy hiPSC-derived CMs and hiPSC-derived NPCs.The alternative text for this image may have been generated using AI.

On the top of both panels, representative confocal images of in situ proximity ligation assay (PLA) performed in hiPSC-CMs (left panel) and hiPSc-NPCs (right panel) using an anti-frataxin antibody in combination with antibodies against the respiratory chain complexes I, II or III. Scale bar: 30 μm. Nuclei were counterstained with DAPI (blue). Red fluorescent dots were quantified and reported as PLA dots/cell (on the bottom). Analyzed data, reported as the means ± SEM, derived from three independent experiments in which at least five different fields were acquired. Statistical significance was determined using unpaired t-test (*p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 compared to healthy control cell lines).

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