Fig. 2: L1 expression influences collagen levels in PDAC. | Cell Death & Disease

Fig. 2: L1 expression influences collagen levels in PDAC.

From: TGF-β1-mediated downregulation of L1CAM in pancreatic ductal adenocarcinoma drives upregulation of collagen 17A1 and MMP2, facilitating tumor invasiveness and metastasis

Fig. 2: L1 expression influences collagen levels in PDAC.The alternative text for this image may have been generated using AI.

a qPCR analysis for L1 and COL17A1 expression in L3.6pl cells (L1empty, L1KD#1 and L1over). Data are normalized to GAPDH expression. b qPCR analysis for L1 and COL17A1 expression in #354 cells (L1empty, L1KD#1 and L1over). Data are normalized to GAPDH expression. c Flow cytometry quantification of COL17A1 expression in L3.6pl and #354 cells (L1empty, L1KD#1 and L1over). d Quantification of extracellular collagen by Sircol assay in L3.6pl and #354 (L1empty, L1KD#1 and L1over) cells. e Representative immunofluorescence images for Hydroxyprolin (HyP, red) in L3.6pl and #354 PDAC cells (L1empty, L1KD#1 and L1over). The nuclei were stained in blue (DAPI). f Representative histological sections of xenografts derived from L1empty and L1KD#2 L3.6pl cells. Tumor sections were (immuno)stained with Hematoxylin & Eosin (H&E), Sirius Red, Second Armonic Generation (SHG), human COL17A1 and murine COL1A1. The nuclei were stained in blue (DAPI). S stroma, T tumor. g Quantification of collagen content in Sirius Red-stained sections. h Quantification of Collagen Assembly Degree and Collagen Fraction in SHG images. i Schematic representation of conditioned medium (c.m.) experiment. Created with BioRender.com. j Quantification of extracellular collagen by Sircol assay in PSC cells grown for 24 h in the presence of L3.6pl (L1empty and L1KD#1) c.m. k qPCR analysis for COL17A1 expression in PSC cells grown for 24 h in the presence of L3.6pl (L1empty and L1KD#1) c.m. Data are normalized to GAPDH expression. *p < 0.05, **p < 0.005, ***p < 0.0005. l qPCR analysis for COL1A1 expression in PSC cells grown for 24 h in the presence of L3.6pl (L1empty and L1KD#1) c.m. Data are normalized to GAPDH expression. *p < 0.05, **p < 0.005, ***p < 0.0005.

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