Fig. 2: PD-L1 could be lactylated.
From: Lactylation stabilizes PD-L1 to promote tumor immune evasion and cell growth
A Immunoblot (IB) analysis of the anti-PD-L1 immunoprecipitates (IPs) derived from the different cell lines. IgG was used as a negative control. B IB analysis of the anti-Kla IPs derived from the H1975 cells. C Schematic of the N-terminal HA-tag-inserted PD-L1 (HA-ins-PD-L1) plasmid. SP signal peptide, ECD extracellular domain, TM transmembrane domain, ICD intracellular domain (upper). IB analysis of the anti-HA IPs derived from the different cells transfected with HA-ins-PD-L1 (bottom). D Schematic of the C-terminal Flag-tag PD-L1 (PD-L1-Flag) plasmid. (left). IB analysis of the anti-Flag IPs derived from the 293 T cells transfected with PD-L1-Flag (right).
