Fig. 2: D-alanine moieties of Ef.LTA are critical for inflammasome activation. | Cell Death Discovery

Fig. 2: D-alanine moieties of Ef.LTA are critical for inflammasome activation.

From: Butyrate potentiates Enterococcus faecalis lipoteichoic acid-induced inflammasome activation via histone deacetylase inhibition

Fig. 2: D-alanine moieties of Ef.LTA are critical for inflammasome activation.The alternative text for this image may have been generated using AI.

A, B PMA-differentiated THP-1 cells were stimulated with 10 μg/ml of LTAs purified from Streptococcus mutans (Sm.LTA), Streptococcus gordonii (Sg.LTA), E. faecalis (Ef.LTA), Streptococcus pneumoniae (Sp.LTA), Staphylococcus aureus (Sa.LTA), Bacillus subtilis (Bs.LTA) and Lactobacillus plantarum (Lp.LTA) or 0.5 μg/ml of Pam3CSK4 in the presence or absence of NaB (10 mM) for 6 h. After the stimulation, the pro- or active forms of caspase-1 and IL-1β in the culture supernatants (Sup) and pro-IL-1β and β-actin in the cell lysates (Cell) were detected by immunoblotting. C, D PMA-differentiated THP-1 cells were stimulated with dealanylated (Δala)-Ef.LTA and dealanylated/deacylated (Δala/acyl)-Ef.LTA in the presence or absence of NaB for 6 h. C Pro- or active forms of caspase-1 and IL-1β in the culture supernatants (Sup) and pro-IL-1β and β-actin in the cell lysates (Cell) were detected by immunoblotting. D IL-1β expression in the culture supernatants was measured by ELISA. Pam3CSK4 was used as a positive control. The results shown are representative of triplicate experiments.

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