Fig. 2: The effect of BCar on the clonogenic survival of breast cancer cells. | Cell Death Discovery

Fig. 2: The effect of BCar on the clonogenic survival of breast cancer cells.

From: Benzimidazole carbamate induces cytotoxicity in breast cancer cells via two distinct cell death mechanisms

Fig. 2: The effect of BCar on the clonogenic survival of breast cancer cells.The alternative text for this image may have been generated using AI.

Log-phase growing breast cancer cells were incubated with BCar at the indicated concentrations for 48 h, washed, and then incubated in a fresh growth medium for two weeks. Colonies were visualized by the crystal violet staining. A Representative samples from clonogenic survival assays. B Colonies in the resulting samples were quantified using ImageJ and analyzed using SigmaPlot software. Results are shown as mean ± s.d. of two sets of experiments in duplicate samples. EC50 values were determined using nonlinear regression with GraphPad Prism software. C Cells morphology photographed using phase-contrast optics. Scale bars represent 100 µm.

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