Fig. 3: BRD7 regulates CHK1 stability and ubiquitination in a USP1-dependent manner. | Cell Death Discovery

Fig. 3: BRD7 regulates CHK1 stability and ubiquitination in a USP1-dependent manner.

From: BRD7 suppresses tumor chemosensitivity to CHK1 inhibitors by inhibiting USP1-mediated deubiquitination of CHK1

Fig. 3: BRD7 regulates CHK1 stability and ubiquitination in a USP1-dependent manner.The alternative text for this image may have been generated using AI.

A Silencing of BRD7 increases USP1 levels. Cells transfected with siRNA targeting BRD7 or scrambled control siRNA were harvested for immunoblotting (IB) with the indicated antibodies. USP1 silencing reverses the effect of BRD7 on CHK1. Cells transfected with the indicated siRNAs were harvested for IB with the indicated antibodies (B), or treated with 100 µg/mL CHX for the indicated time periods and then subjected to IB with the indicated antibodies (C). Densitometry quantification was performed using ImageJ, and the decay curve is shown (right). Data from three independent experiments were expressed as mean ± SEM. D USP1 silencing reverses the effect of BRD7 on CHK1 polyubiquitination. HEK293 cells infected with lentivirus expressing the indicated shRNAs were transfected with the indicated plasmids or siRNAs for 48 h and then treated with 20 µM MG132 for 5 h, followed by Ni-bead pull-down assay and IB with the indicated antibodies. E Silencing of BRD7 enhances the interaction between USP1 and CHK1. H1650 cells transfected with siRNA targeting BRD7 or scrambled control siRNA were lysed for IP with CHK1 antibody or normal IgG, followed by IB with the indicated antibodies. Whole-cell lysates (WCE) were subjected to IB with the indicated antibodies. LEX: longer exposure.

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