Fig. 5: TRPML1 deficiency affects the drug sensitivity of CSCs to salinomycin.
From: Inhibition of lysosomal TRPML1 channel eliminates breast cancer stem cells by triggering ferroptosis
A Representative CSCs proportions assessed by flow cytometry using ALDEFLUOR assay in HCC1954 cells after 48 h treatment of 1 μM Sal and different concentrations of ML-SI1. B Statistics of the CSC proportions in A. C Relative mRNA levels of TRPML1 in scramble control or TRPML1 knockdown (ML1-KD) HCC1954 cells. D Western blot analysis of the TRPML1 levels in scramble control and ML1-KD cells. E Quantification of the TRPML1 levels normalized to ACTB in D. F Mean C11-BODIPY fluorescence intensity in scramble and ML1-KD cells. Cells were pre-incubated with either 1 μM Lip-1 or 20 μM DFO for 48 h in the presence or absence of 5 μM Sal before lipid ROS levels measurement. G Cell viability of scramble and ML1-KD HCC1954 cells pretreated with vehicle or 5 μM Sal for 48 h. H Representative CSC proportions in scramble and ML1-KD HCC1954 cells treated with or without 5 μM Sal for 48 h. I Quantification of the CSC proportions in H. Data are presented as the means ± SEM.
