Fig. 5: Decoding the immune microenvironment of seminomas.

a Bar plot summarizing the proportions of immune cell populations. b UMAP plot highlighting immune cells in metastatic and non-metastatic samples. c Bar plot summarizing the proportions of T cell subpopulations. d UMAP plot highlighting T cell subpopulations in metastatic and non-metastatic samples. e Box plot comparing T cell subpopulation proportions between metastatic and non-metastatic samples. f GSEA plot illustrating the enrichment of an inflammatory response gene signature in non-metastatic T cells. g GSEA plot showing the enrichment of the TNF-α/NF-κB signaling gene signature in non-metastatic T cells. h Violin plot comparing IFNG expression between metastatic and non-metastatic T cells. i Violin plot comparing TNF expression between metastatic and non-metastatic T cells. j Kaplan–Meier survival plot showing that the IFNG+ T cell-associated signature correlates with better PFI. k Kaplan–Meier survival plot showing that the IFNG+ T cell-associated signature correlates with better disease-free interval (DFI). l Bar plot summarizing the proportions of myeloid cell subpopulations in metastatic and non-metastatic samples. m UMAP plot highlighting myeloid subpopulations in metastatic and non-metastatic samples. n GSEA plot showing the enrichment of M2 macrophage signatures in CD163+ macrophages. o GSEA plot showing the enrichment of an inflammatory response gene signature in non-metastatic myeloid cells. p Violin plot comparing CD163 expression between metastatic and non-metastatic myeloid cells. q Violin plot comparing FOLR2 expression between metastatic and non-metastatic myeloid cells. r Kaplan–Meier survival plot showing that the CD163+ macrophage-associated signature correlates with poorer PFI. s Kaplan–Meier survival plot showing that the CD163+ macrophage-associated signature correlates with poorer DFI. t Bubble plot showing cell–cell interactions in non-metastatic samples.