Fig. 6: IFN-γ from IFNG+ T cells induces seminoma differentiation.
From: Single-cell analysis unravels divergent gene signatures shaping seminoma stemness and metastasis
a NicheNet analysis identifying IFNG as a key factor driving differential gene expression between metastatic and non-metastatic seminomas. b UMAP plot illustrating the expression of IFI6 in tumor cells. c UMAP plot showing the expression of IFI35 in tumor cells. d UMAP plot showing the expression of IFIT1 in tumor cells. e UMAP plot showing the expression of IFIT3 in tumor cells. f UMAP plot illustrating the expression of IRF1 in tumor cells. g UMAP plot highlighting the expression of ISG15 in tumor cells. h UMAP plot showing the expression of STAT1 in tumor cells. i Correlation dot plot showing positive association among different genes and pseudotime scores in tumor cells. j Bar plot showing upregulation of IFNG-associated genes following IFN-γ treatment. k Bar plot showing downregulation of stemness-associated genes following IFN-γ treatment. l Overexpression of DPPA4 counteracts the differentiation-inducing effect of IFN-γ. m Overexpression of PSMA7 counteracts the differentiation-inducing effect of IFN-γ. n Schematic model illustrating the molecular and cellular differences between metastatic and non-metastatic seminomas (Created in BioRender. Bian, Z. (2025) https://BioRender.com/b18k646).
