Fig. 5: Further knocking out NLRP3 on the basis of CB2R knockout can alleviate the limitation of skeletal muscle regeneration caused by CB2R knockout. | Cell Death Discovery

Fig. 5: Further knocking out NLRP3 on the basis of CB2R knockout can alleviate the limitation of skeletal muscle regeneration caused by CB2R knockout.

From: Cannabinoid type 2 receptor regulates skeletal muscle regeneration by NLRP3-GSDMD mediated macrophage pyroptosis after injury

Fig. 5: Further knocking out NLRP3 on the basis of CB2R knockout can alleviate the limitation of skeletal muscle regeneration caused by CB2R knockout.The alternative text for this image may have been generated using AI.

a Representative images of GSDMD (red) and F4/80 (green) in Cb2r−/− mice and Cb2r−/−/Nlrp3−/− mice, scale bar = 20 μm. b GSDMD expression levels in macrophages, n = 3, *p < 0.05 Cb2r−/− vs. Cb2r−/−/Nlrp3−/− group. c, d Expression levels of MyoD and Myogenin in regenerated muscle cells of Cb2r−/−/Nlrp3−/−mice and Cb2r−/−/Nlrp3−/− mice, scale bar = 20 μm, n = 3, *p < 0.05 Cb2r−/− vs. Cb2r−/−/Nlrp3−/−group. e, f Western blot and quantitative analysis of eMyHC and Myogenin protein expression levels from whole muscle tissue lysates of mice at 7 days post-injury compared to sham group, n = 3, *p < 0.05 Cb2r−/− vs. Cb2r−/−/Nlrp3−/− group. Data are represented as mean ± SD. ns no significance.

Back to article page