Fig. 3: The presence of a PCNS DLBCL-specific phenotype with a tumor-promoting feature.
From: Single-cell landscape of primary central nervous system diffuse large B-cell lymphoma
a Heatmap of CNV profiles for individual cells (rows) from a representative sample (P201), which were inferred based on the average expression of genes surrounding each chromosomal position (columns). Red: chromosome amplifications; Blue: chromosome deletions. b Clonality tree of single cells from sample P201 based on the results of inferCNV. The branches were scaled according to the percentage of cells in the calculated subclone containing the corresponding CNVs. c UMAP of malignant cells from P201 colored by nodes of the clonality tree in (b). A dashed circle highlights the cells in Node I (including leaf nodes: K, L, O, P). d UMAP of malignant cells from P201 colored by the MP1 signature score (left); violin plot showing the MP1 signature score of cells in the nodes of the clonality tree (right). A dashed circle highlights the cells with high MP1 signature scores. e Stacked bar plot showing proportions of spliced and unspliced gene counts in cells of clonality tree nodes. f Venn diagram showing the DEGs of Node I compared with other nodes only using unspliced and spliced gene counts. Representative DEGs are displayed. UMAP of malignant cells from P201 colored by spliced gene counts (left) and unspliced gene counts (right) for genes SRSF10 (g) and BCL2 (h).
