Fig. 4: A plasmablast-like expression program in PCNS DLBCL.
From: Single-cell landscape of primary central nervous system diffuse large B-cell lymphoma
a Schematic of the workflow for joint analysis of scATAC-seq and scRNA-seq data of B cells. We first mapped the PCA embeddings and cluster annotations of the reference dataset (King et al.) to our scRNA-seq data. Then, cluster annotations were transferred from the scRNA-seq data to the scATAC-seq data through canonical correlation analysis. b UMAP of B cells colored by GC B-cell cluster annotations transferred from publicly available reference data. Dashed circles highlight the plasmablast cells. c UMAP of B cells colored by MP2 signature score. Dashed circles highlight the MP2 cells. d Heatmap showing expression levels of selected functional genes in B cells. e Bar plot showing the TFs that were significantly enriched in plasmablast-like malignant cells. TFs marked with asterisks are the master TFs for differentiation of plasmablast cells. f Strip plot showing the RNA expression levels (upper) and corresponding TF motif activity (bottom) in malignant B cells with GC cluster annotations. g The recurrence-free survival (RFS) curve of 20 PCNS DLBCL patients and the progression-free survival (PFS) curve of 229 systemic DLBCL patients based on MP2 signature scores. The P values were calculated by the log-rank test.
