Fig. 7: TF ELT-1 regulates the 26G pathway by promoting alg-3/4 expression. | Cell Discovery

Fig. 7: TF ELT-1 regulates the 26G pathway by promoting alg-3/4 expression.

From: Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells

Fig. 7: TF ELT-1 regulates the 26G pathway by promoting alg-3/4 expression.The alternative text for this image may have been generated using AI.

a qPCR analysis of alg-3/4 expression in L4-stage C. elegans after knockdown of elt-1. Graphs represent the relative expression to vector RNAi control. ***P < 0.001. b ALG-3/4 levels were analyzed by western blot assay after elt-1 RNAi. Antibodies against GFP and tubulin were used. c Fluorescent micrographs show GFP-tagged ALG-3/4 expression in the germ cells in RNAi control and elt-1 RNAi. Scale bar, 5 μm. d TF ELT-1 binds within the promoters of alg-3/4. e Images showing spermatids before and after activation in vector RNAi control and elt-1 RNAi. The barplot shows statistics of sperm activation. SM: sperm medium. Scale bar, 5 μm. f Volcano plot of 26G-RNAs (defined as 25–27nt, with no first nucleotide bias) on elt-1 RNAi over vector RNAi control. Each dot represents a protein-coding gene. g Illustrations for 26G-RNAs distribution along the genes K09G1.2 and gska-3. 26G-RNAs abundance decreased upon elt-1 RNAi.

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