Fig. 7

Impeding the ILF3–SGOC axis suppresses CRC malignant progression in PDO and PDXs. a Bright-field images and quantification of organoids after 14 days of siRNA transfection. Scale bar, 50 μm. b, c Expression level of ILF3 in indicated patient-derived xenografts (PDXs) (b), and treatment schedule of SGOC inhibitor NCT-503 is indicated (c). The mice were treated with NCT-503 (40 mg/kg/day) for 10 days. d Impact of NCT-503 on tumor growth in mice (n = 7/group) bearing indicated PDXs. The data are presented as the means ± SD. e Representative images of PDX growth monitored by PET-CT. The circles indicate PDXs. f Representative immunofluorescent images for TUNEL⁺ apoptotic signals in PDX tumors and quantitation of apoptotic TUNEL⁺ tumor cells in PDXs after NCT-503 treatment. Scale bars, 50 μm. TUNEL⁺ cells were counted and presented as a bar graph. The data are presented as the means ± SD. g Impact of indicated treatments on tumor growth of PDXs. The data are presented as the means ± SD. Treatment schedule of cetuximab and/or NCT-503 is indicated.